Alpha-L-Fucosidase in Rat Testis During Sexual Maturity
نویسندگان
چکیده
منابع مشابه
Fucosidosis: Deficiency of Alpha-l-fucosidase in Cultured Skin Fibroblasts
Fucosidosis is a newly recognized inborn error of metabolism involving the accumulation o f fucolipids, fucoglycoproteins, a n d fucornucopolysaccharides. T h r e e families h a v e been reported in the literature (1, 2). 1 T h e enzymic defect in fucosidosis has been established b y Van Hoof and Hers (3) as a specific, nearly complete deficiency of the enzyme ~-L-fucosidase. Thusfar, no report...
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An cu-L-fucosidase has been purified from rat epididymis by fractionation of extracts with ammonium sulfate and subsequent chromatography on ion exchange celluloses. It is a glycoprotein with a molecular weight of 210,000 to 220,000 and dissociates in the presence of mercaptoethanol and guanidine hydrochloride or sodium dodecyl sulfate. The dissociated material behaves as two components with mo...
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The alpha-L-fucosidase from Thermotoga maritima (Tm alpha fuc) was converted into alpha-L-transfucosidase variants by directed evolution. The wild-type enzyme catalyzes oligosaccharide synthesis by transfer of a fucosyl residue from a pNP-fucoside donor to pNP-fucoside (self-condensation) with alpha-(1-->3) regioselectivity or pNP-galactoside (transglycosylation) with alpha-(1-->2) regioselecti...
متن کاملHuman liver alpha-L-fucosidase. Purification, characterization, and immunochemical studies.
Human liver alpha-L-fucosidase has been purified 6300-fold to apparent homogeneity with 66% yield by a two-step affinity chromatographic procedure utilizing agarose epsilon-aminocaproyl-fucosamine. Isoelectric focusing revealed that all six isoelectric forms of the enzyme were purified. Polyacrylamide gel electrophoresis of the purified alpha-L-fucosidase demonstrated the presence of six bands ...
متن کاملAlpha-l-Fucosidase Isoenzyme iso2 from Paenibacillus thiaminolyticus
BACKGROUND α-L-Fucosidases are enzymes involved in metabolism of α-L-fucosylated molecules, compounds with a fundamental role in different life essential processes including immune response, fertilization and development, but also in some serious pathological events. According to the CAZy database, these enzymes belong to families 29 and 95. Some of them are also reported to be able to catalyze...
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ژورنال
عنوان ژورنال: Archives of Andrology
سال: 1988
ISSN: 0148-5016
DOI: 10.3109/01485018808986729